Complexation between Hg(II) and biofilm extracellular polymeric substances: An application of fluorescence spectroscopy

	Complexation between Hg(II) and biofilm extracellular polymeric substances: An application of fluorescence spectroscopy
	Daoyong Zhang; Xiangliang Pan; Khan M.G.Mostofa; Xi Chen; Guijin Mu; Fengchang Wu; Jing Liu; Wenjuan Song; Jianying Yang; Yanli Liu; Qinglong Fu
	2010
发表期刊	Journal of Hazardous Materials
卷号	175 期号:1–3 页码:359-365
摘要	The three-dimensional excitation emission matrix (EEM) fluorescence spectroscopy was employed to investigate the interaction of extracellular polymeric substances (EPS) from natural biofilm with Hg(II). The EEM spectra demonstrated that EPS with molecular weight over 14 kDa had two protein-like fluorescence peaks. The fluorescence intensity at both peaks was strongly dependent on the solution pH in the absence and presence of Hg(II), with the maximal fluorescence intensity at neutral pH. Fluorescence of both protein-like peaks was significantly quenched by Hg(II). The values of conditional stability constants (log K_a = 3.28–4.48) derived from modified Stern–Volmer equation are approximate to those for humic substances and dissolved organic matter (DOM), indicating that fluorescent components in EPS have strong binding capacity for Hg(II). Our findings suggest that EPS from biofilm is a class of important organic ligands for complexation with Hg(II) and may significantly affect the chemical forms, mobility, bioavailability and ecotoxicity of heavy metals in the aquatic environment.
关键词	Extracellular Polymeric Substances Mercury Complexation Fluorescence Quenching Titration
学科领域	环境地球化学
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.gyig.ac.cn/handle/352002/6833
专题	环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文环境地球化学国家重点实验室
推荐引用方式 GB/T 7714	Daoyong Zhang,Xiangliang Pan,Khan M.G.Mostofa,et al. Complexation between Hg(II) and biofilm extracellular polymeric substances: An application of fluorescence spectroscopy[J]. Journal of Hazardous Materials,2010,175(1–3):359-365.
APA	Daoyong Zhang.,Xiangliang Pan.,Khan M.G.Mostofa.,Xi Chen.,Guijin Mu.,...&Qinglong Fu.(2010).Complexation between Hg(II) and biofilm extracellular polymeric substances: An application of fluorescence spectroscopy.Journal of Hazardous Materials,175(1–3),359-365.
MLA	Daoyong Zhang,et al."Complexation between Hg(II) and biofilm extracellular polymeric substances: An application of fluorescence spectroscopy".Journal of Hazardous Materials 175.1–3(2010):359-365.

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